Islet Graft Retrieval
Islet Graft Retrieval - Related Videos
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
02:48
Nephrectomy in a Porcine Model: A Surgical Procedure to Retrieve Porcine Kidney Graft for Transplantation
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2025
This video demonstrates nephrectomy, the surgical procedure to remove one of the kidneys along with the section of the ureter. The blood vessels to and from the kidney are tied and divided. The excised kidney can be used for transplantation procedures.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
03:48
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
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2025
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
FAQs
Recovered graft tissue can be examined for islet morphology and cellular composition, showing whether the transplanted tissue retains recognizable structural features and which cell types are present. Researchers can also assess vascularization, meaning the extent of blood-vessel development around the graft, and immune-cell infiltration. Together, these findings provide tissue-level evidence of engraftment and graft survival.
The presence and distribution of immune cells within or around recovered graft tissue can indicate an immune response associated with possible rejection. Interpreting this finding alongside graft morphology and vascularization helps distinguish poor tissue integration from damage linked to immune activity. This combined analysis supports investigations into why an islet transplant underperformed or failed.
Vascularization is evaluated because it is one of the structural features used to characterize how a graft has integrated at its transplantation site. Examining blood-vessel development together with islet morphology and cellular composition gives researchers a broader picture of graft condition. These observations can help compare transplantation strategies and interpret differences in graft survival or function.
Tissue analysis does not stand alone: researchers can compare morphology, cellular composition, vascularization, and immune-cell infiltration with measurements of glucose regulation and insulin production. This relationship helps determine whether visible graft changes correspond to preserved or impaired function. The combined evidence is useful for evaluating engraftment and identifying possible contributors to reduced metabolic control.
After the graft-bearing site is surgically excised, the recovered tissue is fixed or otherwise processed for analysis. The selected processing route prepares the specimen for examination of features such as islet morphology, cellular composition, vascularization, and immune-cell infiltration. These steps convert the retrieved transplant site into material suitable for structural, functional, or molecular investigation.
Researchers use this approach when they need direct evidence about the condition of transplanted islet tissue rather than relying only on physiological measurements. It can support studies comparing transplantation strategies, investigating graft survival and engraftment, and examining possible rejection. In medicine, the findings contribute to understanding graft failure and improving cell-based treatments for diabetes.