Semen Analysis

Semen analysis is a laboratory test that evaluates semen and sperm characteristics to assess male reproductive function and help investigate fertility problems. A collected ejaculate is examined after liquefaction for volume, pH, sperm concentration, motility, and morphology, with results interpreted against established reference ranges because these measures can vary between samples. In medicine, the test supports evaluation of infertility, identifies abnormalities such as low sperm count or impaired movement, and can help monitor sperm production after treatment or confirm the effectiveness of vasectomy. Findings are considered alongside medical history, physical examination, and other reproductive tests.

Semen Analysis - Related Videos

Research

JoVE Journal - Biology

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

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Cited by 21 •

2018

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning

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2025

This protocol presents a simple microaspiration technique to isolate viable somatic cells (SCs) from cryopreserved equine semen. Unlike traditional approaches, this technique avoids long in vitro cultures and overcomes sperm contamination, enabling immediate use in somatic cell cloning.

Education

JoVE Core - Anatomy and Physiology

Sperm Structure and Semen Composition

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2024

During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

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Cited by 8 •

2017

Semen liquefaction is required for sperm to be liberated from the seminal gel. This study provides the procedures for collecting semen from the female reproductive tract post-coitus, and measuring semen liquefaction time.

The Examination of Peroxidase-Positive Leukocytes in Semen

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Cited by 3 •

2024

This paper presents an economical and efficient protocol for examining peroxidase-positive leukocytes in semen. With the assistance of a computer-assisted semen analysis (CASA) system, the concentration of peroxidase-positive leukocytes in semen can be obtained within a total of 60 min, effectively improving the efficiency of andrology laboratory and andrologists.

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