Nerve Axotomy
Nerve Axotomy - Related Videos
03:37
Performing Crush Axotomy on the Facial Nerve in a Mouse Model
0 Views •
2025
Source: Olmstead, D. N., et al., Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury. J. Vis. Exp. (2015).This video demonstrates the facial nerve crush procedure in a mouse, where blunt dissection through key landmarks exposes the facial nerve trunk at the stylomastoid foramen. Forceps are then used to compress the nerve trunk, inducing axonal damage and disrupting neuronal signal transmission.
10:11
Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury
0 Views •
Cited by 27 •
2015
We present a surgical protocol detailing how to perform a cut or crush axotomy on the facial nerve in the mouse. The facial nerve axotomy can be employed to study the physiological response to nerve injury and test therapeutic techniques.
03:24
Two-Photon Laser Axotomy: A Method to Injure Axons in Zebrafish Embryos and Observe Axonal Recovery
0 Views •
2023
This video describes the method to injure axons in zebrafish embryos using two photon laser axotomy and observing axonal recovery from injury.
09:18
In vivo Laser Axotomy in C. elegans
0 Views •
Cited by 46 •
2011
A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.
02:50
Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization
0 Views •
2025
This video demonstrates the staining technique of resin-embedded, osmium tetroxide-treated rat peripheral nerve sections using toluidine blue. The stained nerve sections help visualize the fine structures of peripheral nerves.
FAQs
Separating distal degeneration from proximal neuronal responses lets investigators examine injury as two linked biological processes rather than a single event. The distal segment provides a model of structural breakdown and clearance, whereas the proximal neuron reveals survival programs, altered gene expression, and attempts at regrowth. This distinction helps connect cellular injury signals with eventual recovery or failure.
The peripheral and central nervous systems differ in the environment they provide after axonal damage. In peripheral nerves, Schwann cells and immune cells participate in removing damaged material and establishing conditions favorable to regrowth. Central nervous system neurons encounter stronger inhibitory and environmental barriers, making comparable regeneration less likely and making axotomy useful for studying this contrast.
Schwann cells and immune cells are important because they shape what remains around an injured peripheral axon. Their participation in clearing damaged material can influence whether the local environment supports subsequent regrowth. Examining these cellular contributions helps researchers distinguish a neuron’s intrinsic response from the tissue conditions that either facilitate or impede repair after axotomy.
Researchers use Nerve Axotomy models to follow how an injured neuron changes after its axon is disrupted. The models can reveal activation of injury signals, shifts in gene expression, and whether the proximal neuron initiates regeneration. These readouts connect the physical injury event to neuronal survival and help identify responses associated with recovery rather than degeneration.
An axotomy model can be evaluated through several outcomes rather than a single measure. Investigators may examine distal degeneration, neuronal survival, regenerative attempts, and the effectiveness of repair strategies. Considering these outcomes together shows whether a response merely limits damage, preserves the neuron, or also supports restoration of a failed nervous-system connection.
Within neuroscience, Nerve Axotomy provides an experimental framework for studying why nervous-system connections fail or recover after traumatic nerve injury. Findings can guide investigations of repair strategies by showing which injury responses accompany degeneration, survival, or regrowth. The peripheral-versus-central comparison is especially relevant when assessing why successful repair in one setting may not translate to another.