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Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury
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Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury

Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury

DOI:
10.3791/52382-v

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10:11 min

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February 23, 2015

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, , , , ,
1Anatomy and Cell Biology,Indiana University School of Medicine, 2Research and Development Services,Richard L. Roudebush VA Medical Center, 3Department of Anatomy and Cell Biology,University of Illinois, Chicago

Chapters

  • 00:05Title
  • 01:32Anesthesia and Preparations
  • 03:06Facial Nerve Axotomy: Anatomical Demonstration and Nerve Crush
  • 05:26Facial Nerve Axotomy: Refined Demonstration and Nerve Cut
  • 06:47Closing and Recovery
  • 07:54Results: Facial Nerve Repair and Gene Expression
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

We present a surgical protocol detailing how to perform a cut or crush axotomy on the facial nerve in the mouse. The facial nerve axotomy can be employed to study the physiological response to nerve injury and test therapeutic techniques.

Tags

Keywords: Facial Nerve AxotomyPeripheral Nerve InjuryMotoneuron ResponseFacial ParalysisFacial Motor NucleusLaser MicrodissectionNerve RegenerationGenetically Modified Mice

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