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JoVE Journal

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In vivo Laser Axotomy in C. elegans

In vivo Laser Axotomy in C. elegans

Article DOI: 10.3791/2707
May 19th, 2011


Summary May 19th, 2011

A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.

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