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Preparation of Metabolites from a Coral Holobiont, Separated Coral Host Tissue, and Symbiodiniaceae Cells for Gas Chromatography-Mass Spectrometry Analysis

Metabolite Profiling of Hard Coral Samples Using GC-MS

Article DOI: 10.3791/200438-v • 03:15 min • October 13th, 2023
October 13th, 2023 •
1, 1, 2, 3, 2
1Climate Change Cluster (C3), University of Technology Sydney, 2Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 3School of Biological Sciences, Victoria University of Wellington

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Gas Chromatography-mass Spectrometry Targeted Metabolomics Hard Coral Samples Metabolic Basis Cnidarian-dinoflagellate Symbiosis Temperature-induced Bleaching Steady-state Metabolite Profiling Coral Holobiont Symbiotic Interactions Metabolite Profiles Physical Separation Tissue Isolation Metabolite Extraction GC-MS Analysis Optimization Steps
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