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Cancer Research

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JoVE Journal Cancer Research

Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

Measuring the Motor Aspect of Cancer-Related Fatigue using a Handheld Dynamometer

Journal (Cancer Research)

Measuring the Motor Aspect of Cancer-Related Fatigue using a Handheld Dynamometer

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE

Journal (Cancer Research)

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE

Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

Article DOI: 10.3791/200793-v • 04:00 min • October 27th, 2023

October 27th, 2023 •

Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology, University of Kansas Cancer Center, ²Department of Cancer Biology, University of Kansas Cancer Center, ³BioEngineering Program, University of Kansas, ⁴Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine, University of Kansas Medical Center

* These authors contributed equally

Tags

Rapid In Vitro Cytotoxicity Evaluation Jurkat Chimeric Antigen Receptor Fluorescent Imaging CAR T Cells Targeting Domain Single Chain Variable Fragment Intra-chain Linker Hinge Domain Transmembrane Domain Costimulatory Domain CAR Construct CAR-mediated Killing CAR-T Cells Time-consuming Process Expensive Process Testing Constructs Time And Material Investment Platform Hinge-optimized CAR Constructs Jurkat Cells Lentivirus Uptake CAR Transduction Fluorescent Imager Cytolysis PBMC-derived T Cells

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