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Transmembraan domein oligomerisatie Propensity bepaald door ToxR Assay

Article DOI: 10.3791/2721-v • 06:46 min • May 26th, 2011
May 26th, 2011 •
1, 1, 1
1Department of Chemistry and Biochemistry, University of Colorado at Boulder

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Een efficiënte procedure om de oligomerisatie neiging van single-pass transmembraan-domeinen (TMDs) te beoordelen wordt beschreven. Chimère eiwitten bestaande uit de TMD gefuseerd aan ToxR worden uitgedrukt in een E. coli stam verslaggever. TMD-geïnduceerde oligomerisatie oorzaken dimerisatie van ToxR, activatie van transcriptie en de productie van de reporter eiwit-galactosidase.

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Cellular Biology transmembraan domein oligomerisatie transcriptionele verslaggever ToxR latent membraaneiwit-1
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