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Optische kaart brengen van Intra-sarcoplasmareticulum Ca

Article DOI: 10.3791/53166-v • 09:26 min • September 10th, 2015
September 10th, 2015 •
1, 2, 1
1Department of Pharmacology, University of California Davis, 2Departments of Pharmacology and Biomedical Engineering, University of California Davis

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Dit artikel beschrijft de gedetailleerde protocol en de apparatuur die nodig is voor dual optische kaart brengen van transmembraan potentiaal (V m) en gratis intra-sarcoplasmatisch reticulum (SR) Ca 2+ in de Langendorff-doorbloed konijn hart. Deze methode maakt directe observatie en kwantificering van Vm en SR Ca 2+ dynamiek in het intacte hart.

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Molecular Biology optische mapping sarcoplasmatisch reticulum calcium calcium-gevoelige kleurstof spanningsgevoelige kleurstof aritmie cardiale elektrofysiologie excitatie-contractie koppeling konijn
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