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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Article DOI: 10.3791/2809-v • 15:32 min • August 5th, 2011
August 5th, 2011 •
*1, *1, *1, *1, 1, 1
1Department of Pediatrics and Department of Cancer Biology, University of Massachusetts Medical School
* These authors contributed equally

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A flow-cytometric method for identification and molecular analysis of differentiation-stage-specific murine erythroid progenitors and precursors, directly in freshly –harvested mouse bone marrow, spleen or fetal liver. The assay relies on cell-surface markers CD71, Ter119, and cell size.

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Identification Analysis Mouse Erythroid Progenitors CD71/TER119 Flow-cytometric Assay Erythropoiesis Red Cells Hematopoietic Progenitors Erythropoietin Tissue Hypoxia Erythropoietic Stress Response Circulating Red Cells Tissue Oxygen Delivery Erythropoietic Stress Response Physiological Conditions Pathological Conditions Mouse Model Definitive Erythropoiesis Fetal Liver Neonatal Spleen Adult Spleen Bone Marrow
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