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Neuroscience

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JoVE Journal Neuroscience
Screening Assay voor oxidatieve stress in een Feline astrocyten cellijn, G355-5
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Journal (Neuroscience)
Three-Dimensional Shape Modeling and Analysis of Brain Structures
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
 
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Screening Assay voor oxidatieve stress in een Feline astrocyten cellijn, G355-5

Article DOI: 10.3791/2841-v • 09:53 min • July 13th, 2011
July 13th, 2011 •
1, 1, 1, 2, 3, 2, 1,2
1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products

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Een screening methode om oxidatieve cellulaire omgevingen te detecteren is het meten van de oxidatie van CM-H2DCFDA. Zodra geoxideerd in een cel, CM-H2DCFDA veranderingen ten opzichte van niet-fluorescerende in een fluorescerende verbinding. Deze verandering in fluorescentie wordt gemeten door middel van flowcytometrie en geeft het aantal cellen in een oxidatieve omgeving.

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Neurowetenschappen astrocyten oxidatieve stress flowcytometrie CM-H2DCFDA
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