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Immunology and Infection

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Zuivering en Visualisatie van Lipopolysaccharide van Gram-negatieve bacteriën door Hot Waterige-fenolextractie

Article DOI: 10.3791/3916-v • 05:32 min • May 28th, 2012
May 28th, 2012 •
1, 1
1Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

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Wij beschrijven een gemodificeerd hot waterige fenol extractie werkwijze voor het zuiveren lipopolysaccharide (LPS) van Gram-negatieve bacteriën. Afgetapt kan de LPS vervolgens geanalyseerd door SDS-PAGE en gevisualiseerd door directe kleuring of Western immunoblot.

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Immunologie Gram-negatieve LPS extractie polysaccharide vlekken West-immunoblot
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