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Temporal Sporing af cellecyklusprogression anvendelse af flowcytometri uden behov for synkronisering

Article DOI: 10.3791/52840-v • 08:52 min • August 16th, 2015
August 16th, 2015 •
1, 1
1Centre for Cancer Research, Westmead Millennium Institute for Medical Research and University of Sydney

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Denne protokol beskriver anvendelsen af ​​bromdeoxyuridin (BrdU) -optagelse at tillade den tidsmæssige sporing af celler, der var i S-fase på et bestemt tidspunkt. Tilsætning af DNA farvestoffer og antistof mærkning letter detaljeret analyse af skæbne S faseceller på senere tidspunkter.

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Cellular Biology akut lymfoblastisk leukæmi Celle cyklus bromdeoxyuridin flowcytometri kemoterapeutiske midler Cell synkronisering
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