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Journal / Genetics / Optogenetic यादृच्छिक mutagenesis में…
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Genetics

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Optogenetic यादृच्छिक mutagenesis में Histone-miniSOG का प्रयोग
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Optogenetic यादृच्छिक mutagenesis में Histone-miniSOG का प्रयोग

Article DOI: 10.3791/54810-v • 04:51 min • November 14th, 2016
November 14th, 2016 •
1, 1,2
1Division of Biological Sciences, Section of Neurobiology, University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine, University of California in San Diego School of Medicine and Howard Hughes Medical Institute

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आनुवंशिक रूप से इनकोडिंग हिस्टोन-miniSOG एक नीली बत्ती पर निर्भर ढंग से जीनोम चौड़ा पैतृक म्यूटेशन लाती है। इस mutagenesis विधि सरल, तेज, जहरीले रसायनों से मुक्त है, और आगे आनुवंशिक स्क्रीनिंग और transgene एकीकरण के लिए अच्छी तरह से अनुकूल है।

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आनुवंशिकी अंक 117 जीनोम चौड़ा यादृच्छिक mutagenesis डीएनए संशोधन आरओएस हिस्टोन-miniSOG transgene एकीकरण गुणसूत्र विलोपन आगे आनुवंशिक स्क्रीनिंग
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