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Bioengineering

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JoVE Journal Bioengineering
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Microinjection de Western chrysomèle des racines du maïs, Diabrotica virgifera virgifera, embryons de Transformation de la lignée germinale, ou d’édition de génome CRISPR/Cas9

Article DOI: 10.3791/57497-v • 07:42 min • April 27th, 2018
April 27th, 2018 •
1, 1, 1, 1, 1
1Department of Entomology and Plant Pathology, North Carolina State University

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Nous présentons ici des protocoles pour la collecte et microinjecting maïs precellular embryons de la chrysomèle des racines du afin d’effectuer des analyses génomiques fonctionnelles telles que la transformation de la lignée germinale et CRISPR/Cas9-génome édition.

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Bio-ingénierie numéro 134 microinjection embryonnaire génomique fonctionnelle transformation de la lignée germinale transgénèse CRISPR/Cas9 génome édition la chrysomèle du maïs
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