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用化学干燥进行扫描电镜 (sem) 形态分析的原核生物和真核生物的制备
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Biologia
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JoVE Journal Biologia
Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
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用化学干燥进行扫描电镜 (sem) 形态分析的原核生物和真核生物的制备

DOI:
10.3791/58761-v

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09:58 min

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January 07, 2019

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, , , , ,
1Department of Biology,Central Michigan University

Capitoli

  • 00:04Titolo
  • 00:45Fixation, Washing and Dehydration of Cyanobacteria and Euglenoids
  • 02:36Drying and Mounting
  • 03:54Fixation, Washing and Dehydration of Drosophila
  • 04:55Drosophila Drying and Mounting
  • 06:48Sputter Coating and Imaging
  • 07:20Results: Morphological Analysis of Different Organisms Using SEM Images
  • 09:20Conclusion

Summary

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Traduzione automatica

扫描电镜分析是帮助物种鉴定或表型鉴别的有效方法。该协议描述了检查三种具有代表性的生物体的具体形态细节的方法, 并将广泛适用于检查许多组织和组织类型的特征。

Tags

Scanning Electron MicroscopySEMChemical DryingHexamethyldisilazaneT-butyl AlcoholProkaryotic OrganismsEukaryotic OrganismsCyanobacteriaEuglenoidsFiltrationDehydrationFixationPhosphate BufferEthanol SeriesChemical Drying

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