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Journal / Cancer Research / Gjær som et chassis for å utvikle funksjonelle…
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Cancer Research
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JoVE Journal Cancer Research
Gjær som et chassis for å utvikle funksjonelle analyser for å studere Human P53
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Gjær som et chassis for å utvikle funksjonelle analyser for å studere Human P53

Article DOI: 10.3791/59071-v • 14:57 min • August 4th, 2019
August 4th, 2019 •
1, 2, 3, *3, *1, *2
1Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino, 2Department CIBIO, University of Trento, 3LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto
* These authors contributed equally

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Presentert her er fire protokoller for å konstruere og utnytte gjær Saccharomyces cerevisiae reporter stammer å studere menneskelig P53 transactivation potensial, virkninger av sine ulike kreft-tilknyttede mutasjoner, co-uttrykt samspill proteiner, og virkningene av spesifikke små molekyler.

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Kreftforskning P53 gjær respons element funksjonell analysen transkripsjon veksthemming
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