Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Fremstilling af mitokondrier fra æggestokkene…
JoVE Journal
Cancer Research

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Cancer Research
Fremstilling af mitokondrier fra æggestokkene Cancer væv og kontrol ovarie væv til kvantitativ proteomics analyse
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
Journal (Cancer Research)
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
Analyzing Fluorescence in Lung Cancer Tissue Samples Stained with Optimized Multiplex Immunohistochemistry (mIHC) Technique
Journal (Cancer Research)
Analyzing Fluorescence in Lung Cancer Tissue Samples Stained with Optimized Multiplex Immunohistochemistry (mIHC) Technique
 
Click here for the English version

Fremstilling af mitokondrier fra æggestokkene Cancer væv og kontrol ovarie væv til kvantitativ proteomics analyse

Article DOI: 10.3791/60435-v • 11:51 min • November 18th, 2019
November 18th, 2019 •
1,2,3,4, 5, 1,2,3, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 5Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne artikel præsenterer en protokol med differentialhastighed centrifugering i kombination med tæthed gradient centrifugering at adskille mitokondrier fra humane æggestokkene Cancer væv og kontrol ovarie væv til kvantitativ proteomics analyse, resulterer i en høj kvalitet mitokondrie prøve og høj gennemløb og høj reproducerbarhed kvantitativ proteomics analyse af en menneskelig ovariecancer mitokondrie proteome.

Tags

Kræftforskning kræft i æggestokkene mitokondrier differentialhastighed centrifugering tæthed gradient centrifugering isobarisk tag for relative og absolutte kvantificering (itraq) mærkning stærk kation udveksling (SCX) væskekromatografi (LC) tandem massespektrometri (MS/MS) mitokondrie proteom
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter