Rivista
/
Biologia
/
测量小鼠分离和孵育成熟骨骼肌中胰岛素和收缩刺激的葡萄糖摄取
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
Please note that all translations are automatically generated. Click here for the English version.

测量小鼠分离和孵育成熟骨骼肌中胰岛素和收缩刺激的葡萄糖摄取

DOI:
10.3791/61398-v

•

08:01 min

•

May 16, 2021

•

, , , , , ,
1Section of Molecular Physiology, Department of Nutrition, Exercise and Sports,University of Copenhagen, 2Section of Integrative Physiology, Department of Nutrition, Exercise and Sports,University of Copenhagen

Capitoli

  • 00:04Introduction
  • 00:39Mouse Soleus and Extensor Digitorum Longus (EDL) Harvest
  • 03:15Insulin-Stimulated Glucose Uptake
  • 04:18Contraction-Stimulated Glucose Uptake
  • 04:57Skeletal Muscle Homogenization and Radiolabeled 2-Deoxyglucose and Mannitol Determination
  • 05:58Results: Representative Mouse Soleus and EDL Muscle Metabolic Activity Analysis
  • 07:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

肌肉葡萄糖摄取的完全调节对于维持全身葡萄糖稳态非常重要。该方案在描述各种生理干预对全身葡萄糖代谢的影响时，评估孤立和孵育成熟骨骼肌中胰岛素和收缩刺激的葡萄糖摄取。

Tags

Glucose UptakeSkeletal MuscleInsulinContractionEx Vivo ModelMouseSoleusTibialis AnteriorEDLIncubationDissectionSuture LoopsForced TransducersBasal Incubation MediumOxygenCarbon Dioxide

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

在体内微循环在骨骼肌内重要的显微镜测量

成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离

骨骼肌的线粒体分离

纯化的骨骼肌的先觉者

基于荧光测定活细胞中的钙进入商店经营：从培养的肿瘤细胞，骨骼肌纤维

体外评估对离体骨骼肌的收缩，疲劳性和交替“

单个肌纤维和成人骨骼肌卫星细胞的分离和培养

从人骨骼肌隔离血管源性多能前体

平滑肌功能中分离出来的组织浴，应用药理学研究测量

葡萄糖摄取测量和对胰岛素刺激的反应<em>体外</em>培养的人类初级肌管

Read Article