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Journal / Bioengineering / Schnelle Verkapselung des rekonstituierten…
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Bioengineering

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JoVE Journal Bioengineering
Schnelle Verkapselung des rekonstituierten Zytoskeletts in riesigen unilamellären Vesikeln
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Schnelle Verkapselung des rekonstituierten Zytoskeletts in riesigen unilamellären Vesikeln

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Dieser Artikel stellt eine einfache Methode zur schnellen Herstellung von riesigen unilamellären Vesikeln mit verkapselten zytoskelettalen Proteinen vor. Die Methode erweist sich als nützlich für die Bottom-up-Rekonstitution von Zytoskelettstrukturen in Enstrikt- und Zytoskelett-Membran-Interaktionen.

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Bioengineering Ausgabe 177 Emulsionstransfer cDICE GUVs Bottom-up-Rekonstitution Aktin Faschin
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