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Journal / Cancer Research / 合并的shRNA文库筛选，以确定调节耐药性表型的因素…
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JoVE Journal Cancer Research
合并的shRNA文库筛选，以确定调节耐药性表型的因素
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合并的shRNA文库筛选，以确定调节耐药性表型的因素

Article DOI: 10.3791/63383-v • 14:51 min • June 17th, 2022
June 17th, 2022 •
1,2, 1,2, 2,3, 2,3, 1,2,3, 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

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使用慢病毒 shRNA 池的高通量 RNA 干扰 （RNAi） 筛选可以成为检测恶性肿瘤中治疗相关合成致死靶标的工具。我们提供一种合并的shRNA筛选方法来研究急性髓系白血病（AML）中的表观遗传效应子。

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癌症研究，第184期，高通量筛选，RNAi筛选，shRNA，表观遗传因子，阿糖胞苷，AraC耐药性
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