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Genetics

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Præimplantationsgenetisk test for aneuploidi på en halvlederbaseret næste generations sekventeringsplatform

Article DOI: 10.3791/63493-v • 09:30 min • August 17th, 2022
August 17th, 2022 •
*1, *2, 1, 1, 3, 4, 5, 1, 1, 1, 2, 2, 1
1Centre for Women, Children, and Reproduction, Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 3Department of Obstetrics and Gynecology, Chinese PLA General Hospital, 4Clinical Laboratory, Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
* These authors contributed equally

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Protokollen præsenterer de overordnede procedurer i laboratoriet, der kræves i genetisk test før implantation for aneuploidi på en halvlederbaseret næste generations sekventeringsplatform. Her præsenterer vi de detaljerede trin i helgenomforstærkning, udvælgelse af DNA-fragment, bibliotekskonstruktion, skabelonforberedelse og sekventering af arbejdsflow med repræsentative resultater.

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