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Journal / Neuroscience / 현장 Cryosectioned Zebrafish 배아에서 면역조직화학과 결합된…
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Neuroscience

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JoVE Journal Neuroscience
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현장 Cryosectioned Zebrafish 배아에서 면역조직화학과 결합된 혼성화

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

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이 프로토콜은 제브라피쉬 배아 절편의 현장 혼성화와 면역조직화학을 결합하여 이미지를 얻는 방법을 설명합니다. 저온 절개 전에 제자리 하이브리드화를 수행한 후 항체 염색을 수행했습니다. 항체가 부족한 경우 제브라피쉬에서 두 유전자의 발현 패턴을 감지하는 것이 유용합니다.

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