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Karakterisera RNA-modifieringar i enskilda neuroner med hjälp av masspektrometri
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Karakterisera RNA-modifieringar i enskilda neuroner med hjälp av masspektrometri

Article DOI: 10.3791/63940-v • 08:45 min • April 21st, 2022
April 21st, 2022 •
1,2, 1,2, 1,2
1Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 2Department of Chemistry, University of Illinois Urbana-Champaign

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Post-transkriptionella modifieringar av RNA representerar ett understuderat lager av översättningsreglering som nyligen har kopplats till centrala nervsystemets plasticitet. Här beskrivs provberedning och vätskekromatografi-tandemmasspektrometrimetod för samtidig karakterisering av många RNA-modifieringar i enskilda neuroner.

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Kemi Utgåva 182 RNA-modifiering encellig vätskekromatografi masspektrometri nukleosider neuron Aplysia californica epitranscriptom
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