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Karakterisering af RNA-modifikationer i enkeltneuroner ved hjælp af massespektrometri
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Karakterisering af RNA-modifikationer i enkeltneuroner ved hjælp af massespektrometri

Article DOI: 10.3791/63940-v • 08:45 min • April 21st, 2022
April 21st, 2022 •
1,2, 1,2, 1,2
1Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 2Department of Chemistry, University of Illinois Urbana-Champaign

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Post-transkriptionelle modifikationer af RNA repræsenterer et understuderet lag af translationsregulering, der for nylig er blevet forbundet med centralnervesystemets plasticitet. Her beskrives prøveforberedelse og væskekromatografi-tandem massespektrometri tilgang til samtidig karakterisering af talrige RNA-modifikationer i enkelte neuroner.

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Kemi udgave 182 RNA-modifikation enkeltcelle væskekromatografi massespektrometri nukleosider neuron Aplysia californica epitranscriptom
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