JoVE Journal
Developmental Biology
Developmental Biology
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流式细胞术从小鼠组织中鉴定和分离爆破形成单元和集落形成单元红系祖细胞
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Article
DOI:
10.3791/64373-v
•
08:31 min
•
November 4th, 2022
流式细胞术从小鼠组织中鉴定和分离爆破形成单元和集落形成单元红系祖细胞
November 4th, 2022
•
Capitoli
Riepilogo
Please note that all translations are automatically generated.
Click here for the English version.
在这里,我们描述了一种新的流式细胞术方法,用于直接从新鲜小鼠骨髓和脾脏中前瞻性分离早期爆发形成单位红系(BFU-e)和集落形成单位红系(CFU-e)祖细胞。该协议基于单细胞转录组学数据开发,是第一个以高纯度分离所有组织红系祖细胞的方案。