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JoVE Journal
Developmental Biology

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流式细胞术从小鼠组织中鉴定和分离爆破形成单元和集落形成单元红系祖细胞
 
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流式细胞术从小鼠组织中鉴定和分离爆破形成单元和集落形成单元红系祖细胞

Article DOI: 10.3791/64373-v 08:31 min November 4th, 2022
November 4th, 2022

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在这里,我们描述了一种新的流式细胞术方法,用于直接从新鲜小鼠骨髓和脾脏中前瞻性分离早期爆发形成单位红系(BFU-e)和集落形成单位红系(CFU-e)祖细胞。该协议基于单细胞转录组学数据开发,是第一个以高纯度分离所有组织红系祖细胞的方案。

Tags

发育生物学,第189期,红细胞生成,流式细胞术,FACS,CFU-e,BFU-e,红系祖细胞
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