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Populations- og enkeltcelleanalyse af antibiotikapersistens i Escherichia coli
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Populations- og enkeltcelleanalyse af antibiotikapersistens i Escherichia coli

Article DOI: 10.3791/64550-v • 12:29 min • March 24th, 2023
March 24th, 2023 •
*1, *1, 1, 1
1Bacterial Genetics and Physiology, Département de Biologie Moléculaire, Faculté des Sciences, Université Libre de Bruxelles (ULB)
* These authors contributed equally

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Antibiotisk persistens beskriver små subpopulationers evne inden for en følsom isogen population til forbigående at tolerere høje doser bakteriedræbende antibiotika. Denne protokol kombinerer tilgange til at karakterisere antibiotisk persistensfænotype på molekylært og cellulært niveau efter udsættelse af Escherichia coli for dødelige doser ofloxacin.

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Biologi udgave 193 Antibiotikapersistens populationsanalyse enkeltcelleanalyse Escherichia coli ofloxacin fluorescensmikroskopi mikrofluidik
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