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Enriquecimiento de cultivos de neuronas de ganglios de la raíz dorsal de ratones adultos mediante inmunopanización
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Enriquecimiento de cultivos de neuronas de ganglios de la raíz dorsal de ratones adultos mediante inmunopanización

Article DOI: 10.3791/64603-v • 08:57 min • February 24th, 2023
February 24th, 2023 •
1, 1,2,3, 1,4,5
1Neuroscience and Mental Health Institute, University of Alberta, 2Department of Medicine, Division of Neurology, University of Alberta, 3Department of Medical Microbiology and Immunology, University of Alberta, 4Department of Pharmacology, University of Alberta, 5Department of Anesthesiology and Pain Medicine, University of Alberta

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Este artículo describe un protocolo de inmunopanopanización para los ganglios de la raíz dorsal del ratón adulto. Al adherir anticuerpos a las placas de cultivo, podemos seleccionar y eliminar negativamente las células no neuronales. Mostramos que los cultivos se enriquecen para las neuronas utilizando este protocolo, lo que permite un estudio en profundidad de las respuestas neuronales a la manipulación.

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Retractación Número 192
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