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Establecimiento y cultivo de organoides mamarios derivados de pacientes

Article DOI: 10.3791/64889-v • 08:29 min • February 17th, 2023
February 17th, 2023 •
1,2, 1, 1, 1, 1,2
1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University

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Aquí se proporciona un protocolo detallado para establecer organoides mamarios humanos a partir de resecciones de tumores de mama derivados de pacientes o tejido mamario normal. El protocolo proporciona instrucciones completas paso a paso para el cultivo, la congelación y la descongelación de organoides mamarios derivados de pacientes humanos.

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Retractación Número 192
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