Exposure Culture Method
Exposure Culture Method - Related Videos
11:09
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
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Cited by 9 •
2016
Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 41 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
08:45
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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Cited by 9 •
2008
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
02:52
Assessing the Antibacterial Activity of Nanostructured Surfaces Using a Focused-Contact Exposure Method
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2026
Source: Holt-Torres, P. S. et al. Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro. J. Vis. Exp. (2023)This video demonstrates the use of the focused-contact exposure method to evaluate the antibacterial activity of nanostructured surfaces. It outlines the steps involved in establishing direct contact between bacteria and the nanostructured surface, incubation, bacterial collection, and colony counting to assess viability.
09:08
Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
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Cited by 11 •
2016
In the current climate of scarce resources, new technologies are emerging that allow researchers to conduct studies cheaper, faster and with more precision. Here we describe the development of a bead-based salivary antibody multiplex immunoassay to measure human exposure to multiple environmental pathogens simultaneously.
FAQs
Controlled variation of concentration and duration allows researchers to connect an exposure level with a biological response. Holding other factors, such as temperature and culture conditions, stable makes dose-response relationships easier to interpret, while changing delivery route can show whether the response depends on how the exposure reaches the cells or engineered system. This design helps distinguish weak, moderate, and damaging conditions.
These variables can alter how cells, tissues, or engineered systems experience an exposure. Delivery route affects contact with the biological material, while temperature and other culture conditions may influence the resulting response. Regulating them reduces unwanted variation and makes comparisons between experimental conditions more meaningful, particularly when researchers evaluate whether an observed change reflects the exposure itself rather than an uncontrolled culture factor.
Viability, morphology, function, and molecular activity provide complementary evidence about biological response. Viability indicates whether cells or tissues remain alive, whereas morphology can reveal structural changes. Functional measurements show whether performance has changed, and molecular activity can help identify underlying responses. Considering these readouts together helps researchers distinguish general damage from functional adaptation or more specific exposure-related mechanisms.
A typical workflow selects the cultured cells, tissue, or engineered system, defines the exposure type and experimental conditions, and regulates concentration, duration, delivery route, temperature, and culture environment. Researchers then apply the exposure and measure selected biological outcomes, such as viability, morphology, function, or molecular activity. Comparing those measurements across controlled conditions supports interpretation of response patterns and dose effects.
The method is useful when a biomaterial or therapeutic candidate must be examined under defined biological conditions before broader development. Researchers can expose cultured cells, tissues, or engineered systems to the candidate and quantify resulting changes. The findings may reveal harmful responses, functional effects, or concentration-dependent behavior, helping guide the design of safer medical technologies and the selection of conditions for further study.
In bioengineering, controlled exposure experiments connect engineered materials or tissue systems with measurable biological outcomes. They can be applied to biomaterials, therapeutic candidates, environmental hazards, and engineered tissue responses. By comparing viability, morphology, function, or molecular activity under defined conditions, researchers obtain evidence that can inform safer medical and industrial technologies while clarifying how engineered systems respond to chemical, biological, or physical challenges.