Vion Ims Qtof
Vion Ims Qtof - Related Videos
11:09
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
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Cited by 9 •
2018
Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.
16:40
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
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Cited by 51 •
2010
Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.
16:57
MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease
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Cited by 57 •
2012
Dopamine replacement pharmacotherapy using L-DOPA is the most commonly used symptomatic treatment of Parkinson’s disease, but is accompanied by side effects including involuntary abnormal movements, termed dyskinesia 1. Here, a protocol for MALDI imaging mass spectrometry is presented that detects changes in rat brain neuropeptide levels related to dyskinesia.
09:45
In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
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Cited by 7 •
2020
Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.
08:38
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
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Cited by 6 •
2011
Testing protein-protein interaction is indispensable for dissection of protein functionality. Here, we introduce an in vitro protein-protein binding assay to probe a membrane-immobilized protein with a soluble protein. This assay provides a reliable method to test interaction between an insoluble protein and a protein in solution.
FAQs
These properties determine how readily an ion moves through the gas-filled mobility cell under an electric field. Ions with different physical forms or charge states can therefore follow different mobility behaviors even when their mass-to-charge values are similar. This separation adds structural information and helps distinguish chemical species that mass analysis alone may not resolve.
Mass-to-charge measurement describes an ion’s mass relationship, whereas mobility provides an additional separation dimension related to its gas-phase behavior. Considering both measurements narrows the possibilities for assigning a compound and helps separate overlapping signals in complex mixtures. The combined information is particularly useful when compounds share similar mass-to-charge ratios or represent structural isomers.
After ions are separated according to their mobility behavior, quadrupole transmission and time-of-flight measurement provide complementary mass information. The quadrupole contributes controlled ion transmission, while the time-of-flight analyzer measures mass and supports accurate-mass data. Together, these stages connect mobility-based separation with compositional characterization for more confident chemical interpretation.
Structural isomers can have the same elemental composition and similar mass-to-charge values while differing in molecular arrangement. Their different gas-phase shapes may produce distinct mobility behavior in the cell. When that mobility information is considered alongside accurate-mass measurements, researchers gain a basis for distinguishing candidate structures that would be difficult to separate using mass information alone.
The measurement begins by ionizing chemical molecules, then introducing the resulting ions into the gas-filled mobility cell under an electric field. Their mobility behavior is recorded before quadrupole transmission and time-of-flight analysis supply mass-to-charge and accurate-mass information. Interpreting these dimensions together produces a multidimensional description of the sample rather than a single mass value.
It is useful when researchers need to identify molecules, examine impurities, analyze metabolites, or investigate molecular structure and composition in mixtures containing chemically similar species. The added mobility dimension can help separate signals that overlap in mass-to-charge space, while accurate-mass data supports compound characterization. These capabilities make the platform relevant to both identification and structural studies.