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JoVE Journal
Biology

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In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
 

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Article DOI: 10.3791/61196-v 09:45 min June 13th, 2020
June 13th, 2020

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Summary

Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.

Transcript

Tags

In Vivo Protein Turnover Aging C. Elegans Photoconvertible Dendra2 Tracking Quantification Degradation Noninvasive Technique Microscopy Set Up Fluorophore Adaptable To Mammalian Systems Zebrafish Proteostasis Network Transport And Trafficking Blue Light Laser Acquisition Settings Fusion Protein Nematodes Imaging Experiment Agarose Glass Slide Confocal Microscope Software Excitation And Emission
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