Lineage Expansion
Lineage Expansion - Related Videos
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
07:48
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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Cited by 15 •
2015
In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.
01:21
Lineage Commitment
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2023
Commitment is the process whereby stem cells: lose their ability to form all cell types and irreversibly change into a specific type. The multipotent hematopoietic stem cells, (HSCs), differentiate into the multipotent hematopoietic progenitor cells, (HPCs). The HPCs express many lineage-specific cytokine receptors. Each of these receptors binds specific cytokines, activates distinct signaling pathways, and expresses a particular gene set. The HPCs further differentiate to form committed...
09:36
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
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Cited by 21 •
2014
Targeting brain-resident cells for direct lineage-reprogramming offers new perspectives for brain repair. Here we describe a protocol of how to prepare cultures enriched for brain-resident pericytes from the adult human cerebral cortex and convert these into induced neurons by retrovirus-mediated expression of the transcription factors Sox2 and Ascl1.
07:35
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Cited by 8 •
2011
This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.
FAQs
Self-renewal preserves the ability of cells in a founder lineage to continue producing descendants, while mitotic proliferation increases the number of daughter cells. Together, these processes allow a lineage to grow while transmitting genetic information from the ancestral cell. This combination makes it possible to examine both the persistence of inherited traits and the emergence of changes during repeated cell divisions.
Daughter cells inherit genetic information from the founder lineage, but descendants may also accumulate genetic or epigenetic changes as the lineage grows. Genetic changes alter inherited sequence information, whereas epigenetic changes can affect how that information is regulated. Tracking both types of change helps explain why cells from one lineage may develop different characteristics or contribute differently to later outcomes.
Lineage expansion focuses on descendants connected to a common ancestral cell or population, so it can reveal the contribution of a particular founder lineage. A broader population increase may combine growth from many unrelated lineages and therefore obscure their individual histories. This distinction allows researchers to separate clonal growth, meaning expansion from one ancestral source, from overall population changes.
Measurements of lineage expansion can show how many descendants arise from a common founder and how their representation changes over time. These patterns help researchers trace cell fate, meaning the developmental outcome adopted by descendants, while also examining whether genetic or epigenetic changes accompany expansion. The resulting information connects cellular proliferation with the formation of distinct biological outcomes.
In genetics, lineage expansion is useful when researchers need to determine how inherited traits or genetic variants spread through cells or populations. Developmental studies can use it to follow descendants as they adopt different fates, while disease research can examine the growth of related cell groups. Evolutionary studies apply the same perspective to connect lineage growth with inherited variation and selection.
Following a lineage connects later cells or organisms to a shared ancestral source, helping researchers compare inherited genetic information with changes that arise during proliferation. If some descendants become more represented, lineage analysis can help examine whether inherited traits, accumulated changes, or selection contributed to that pattern. This supports interpretation of how proliferation and selection shape genetic outcomes across cellular or organismal populations.