Bleomycin Induction
Bleomycin Induction - Related Videos
06:52
Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin
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Cited by 13 •
2019
Here we present an effective method to investigate the antifibrotic activity of intravenously infused human mesenchymal stromal cells obtained from the whole umbilical cord following the induction of lung injury by an endotracheal injection of bleomycin in C57BL/6 mice. This protocol can be easily extended to the preclinical testing of other therapeutics.
05:45
A Refined Aerosol-Based Intratracheal Bleomycin Delivery Method for Reproducible and Minimally Invasive Mouse Models of Pulmonary Fibrosis
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2026
Here, we present a protocol to model pulmonary fibrosis in mice using aerosolized bleomycin delivered via the trachea. This optimized method enables uniform, quantitative, and precise delivery without surgical incision, enhancing the reproducibility and reliability of bleomycin-induced fibrosis.
11:33
Inductance
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN This experiment will use inductive coils to demonstrate the concept of inductor and inductance. Magnetic induction will be demonstrated using a rod magnet inserted into or extracted away from the core of a coil to induce a transient electromotive force (emf) voltage in the coil, measured by a voltmeter. This experiment will also demonstrate the mutual inductance between...
14:01
Anesthesia Induction and Maintenance
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The Guide for the Care and Use of Laboratory Animals ("The Guide") states that pain assessment and alleviation are integral components of the veterinary care of laboratory animals. The definition of anesthesia is the loss of feeling or sensation. It is a dynamic event involving changes in anesthetic depth with respect to an animal's metabolism, surgical stimulation, or variations in the...
11:18
AC Induction Motor Characterization
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2023
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. The objectives of this experiment are to find the equivalent circuit parameters of a three-phase induction motor using the per-phase equivalent circuit and tests similar to those used in transformer characterization. In electrical engineering, an equivalent circuit (or theoretical circuit) can be determined for a given system. The equivalent circuit retains all characteristics of the original system,...
FAQs
After bleomycin exposure, iron-dependent reactive oxygen species first produce DNA strand breaks and tissue injury. These events activate inflammatory signaling and damage epithelial cells. The resulting injury is followed by fibroblast activation and excessive collagen deposition, creating a sequence that links an initial chemical insult to structural tissue remodeling and allows disease progression to be examined experimentally.
Iron is central because bleomycin’s damaging activity depends on iron-dependent generation of reactive oxygen species. Those reactive molecules create DNA strand breaks, providing an initiating signal for downstream injury and inflammation. This mechanism helps researchers connect the agent’s biochemical action with later cellular changes rather than treating fibrosis as an isolated collagen abnormality.
Epithelial damage and fibroblast activation represent different stages and cellular consequences of the response. Injury to epithelial cells contributes to inflammatory signaling, while activated fibroblasts participate in the accumulation of collagen. Examining both processes helps investigators study how tissue damage is converted into remodeling, which is central to understanding fibrotic disease mechanisms.
Investigators use the induced injury response as a controlled setting in which potential antifibrotic treatments can be evaluated. They can examine whether an intervention changes the progression from injury and inflammation toward fibroblast activation and collagen accumulation. This links treatment assessment to the cellular and molecular events underlying tissue remodeling.
A broad study workflow begins by inducing tissue injury with bleomycin, then examining the resulting inflammatory, epithelial, fibroblast, and collagen responses. Investigators can use this sequence to study disease progression and molecular mechanisms before assessing candidate antifibrotic treatments. The design links the initiating injury to remodeling outcomes within a controlled experimental framework.
The model reproduces a controlled injury response, but experimental tissue injury does not fully reproduce human disease. Consequently, findings about inflammatory signaling, epithelial damage, fibroblast activation, or collagen deposition can clarify mechanisms and support treatment evaluation without guaranteeing that the same disease course or therapeutic effect will occur in patients.