Kidney Reperfusion
Kidney Reperfusion - Related Videos
09:09
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
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Cited by 141 •
2013
We describe models of moderate and severe ischemia-reperfusion-induced kidney injury in which the mice undergo unilateral renal pedicle clamping followed by simultaneous or delayed contralateral nephrectomy, respectively. These models consistently give rise to renal dysfunction and post-injury fibrosis, but injury severity and survival are dependent on mouse background, age and surgical equipment.
04:43
Orthotopic Porcine Kidney Transplantation: A Technique to Transplant Graft Kidney in Porcine Model
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2025
This video describes orthotopic kidney auto-transplantation in a porcine model. This surgical procedure involves the transplantation of an animal's kidney graft at its original anatomical location. The model is used to investigate new treatments for end-stage kidney diseases in humans.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
05:36
Kidney Procurement in a Preclinical Large Animal Model
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2025
Here, we present a protocol that details a surgical model for kidney procurement in a preclinical swine model for subsequent machine perfusion or transplantation.
07:29
Intravascular Delivery of Biologics to the Rat Kidney
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Cited by 1 •
2016
The administration of drugs for recovery of kidney function requires control of the localization and distribution of the therapeutic compound. Here, we describe in detail a simple technique for intrarenal delivery of drugs in rats. This procedure may be easily performed with no mortality and high reproducibility.
FAQs
Reintroducing blood flow supplies oxygen and nutrients needed for renal recovery, but the transition can also generate reactive oxygen species. These highly reactive molecules may intensify cellular stress and contribute to tubular cell damage. Consequently, successful restoration of circulation does not automatically guarantee recovery, making the balance between oxygen delivery and reperfusion injury clinically important.
Kidney reperfusion can activate inflammatory pathways after circulation returns to previously ischemic tissue. This inflammatory response adds to the initial effects of reduced blood flow and may amplify renal tissue injury. Understanding that interaction helps explain why treatment strategies aim not only to restore circulation, but also to limit harmful responses that follow reperfusion.
Impaired microvascular function can limit how effectively restored circulation supports renal tissue after kidney reperfusion. Even when blood flow has returned, microvascular abnormalities may interfere with the delivery of oxygen and nutrients within the kidney. This helps account for continued tissue stress and tubular damage despite technically successful restoration of circulation.
Renal function after kidney reperfusion depends on whether the benefits of renewed oxygen and nutrient delivery outweigh damage from reactive oxygen species, inflammation, microvascular impairment, and tubular injury. This balance influences recovery from ischemic injury and helps explain why reperfusion is treated as both a necessary restorative event and a potential source of additional renal damage.
During transplantation, the kidney must recover after a period in which its circulation has been reduced or interrupted. Reperfusion then provides oxygen and nutrients, but it may also provoke oxidative, inflammatory, microvascular, and tubular injury. The resulting balance can influence graft recovery and renal function, which is why organ preservation and protective strategies are important in this setting.
Kidney reperfusion is particularly relevant in transplantation, vascular surgery, and treatment of acute kidney injury. In each context, restoring renal circulation can support recovery from ischemic injury while also creating conditions for reperfusion damage. These settings therefore provide important clinical contexts for evaluating how circulation, tissue injury, and subsequent renal function are related.
Research on kidney reperfusion can guide improvements in organ preservation, surgical strategies, and therapies intended to protect renal tissue from ischemia-reperfusion damage. The key focus is the interaction between restored circulation and harmful downstream effects, including reactive oxygen species, inflammation, microvascular dysfunction, and tubular cell injury. Such knowledge can support efforts to improve renal recovery.