Vascularization Assessment
Vascularization Assessment - Related Videos
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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Cited by 7 •
2014
The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.
06:55
Assessment of Maternal Vascular Remodeling During Pregnancy in the Mouse Uterus
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Cited by 27 •
2015
This protocol describes a technique to assess changes in the maternal vasculature during pregnancy in mice. Using stereological methods, remodeling of the decidual spiral arteries is assessed quantitatively and the results confirmed qualitatively using immunohistochemistry.
08:50
Assessment of Vascular Function in Patients With Chronic Kidney Disease
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Cited by 25 •
2014
The degree of vascular dysfunction and contributing physiological mechanisms can be assessed in patients with chronic kidney disease by measuring brachial artery flow-mediated dilation, aortic pulse-wave velocity, and vascular endothelial cell protein expression.
02:43
Assessing Mitochondrial Function in Cerebral Vascular Endothelial Cells
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2025
This video demonstrates the assessment of the mitochondrial functioning of cerebral vascular endothelial (CVE) cells using an extracellular flux assay. CVE cells are plated in an extracellular flux cell culture plate. Extracellular flux sensors are hydrated before loading and calibration into the bioanalyzer. The plate containing cells is then loaded for analysis.
09:33
A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology
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Cited by 16 •
2015
The present article describes the methodological considerations for several non-invasive assessments of vascular function and morphology that are commonly used in medical research to assess different stages of atherosclerosis.
FAQs
These measurements describe related but distinct aspects of tissue perfusion. Vessel density indicates how extensively vessels are present, while blood flow reflects circulation through them and oxygen delivery addresses the supply reaching tissue. Considering these features together can provide a more informative picture of tissue viability than relying on a single vascular indicator.
A tissue may contain blood vessels without receiving adequate circulating blood or oxygen. Assessing structural features alongside functional measures helps distinguish the presence of vessels from their contribution to tissue support. This distinction is relevant when evaluating circulation, tissue viability, healing, tumors, or transplanted and engineered tissues.
Clinical examination and imaging-based measurements offer complementary ways to evaluate vascular status. Examination can support assessment in a clinical setting, whereas imaging can provide measurements of features such as vessel density or blood flow. The selected approach should match the tissue, the clinical or research question, and the information needed for interpretation.
A typical assessment begins by identifying the tissue or organ and the vascular question being investigated. The evaluator then selects clinical examination or imaging-based measurement, examines relevant features such as vessel density, blood flow, or oxygen delivery, and interprets the findings in relation to tissue viability, circulation, or treatment response.
It is particularly useful when impaired circulation, wound healing, tumor characteristics, or the status of transplanted or engineered tissue must be evaluated. Findings can contribute to diagnosis and treatment planning, while repeated assessments can help determine whether tissue condition or therapeutic outcomes are changing over time.
In research, these evaluations provide a way to examine how blood vessel development relates to regenerative medicine and tissue repair. Measuring vascular features can help characterize engineered tissues, assess their viability, and evaluate therapeutic outcomes. The same approach also supports investigation of angiogenesis, the process associated with developing vascular supply during repair studies.