Acute Slices
Acute Slices - Related Videos
09:10
Preparation of Acute Subventricular Zone Slices for Calcium Imaging
0 Views •
Cited by 2 •
2012
A method to load subventricular zone (SVZ) cells with calcium indicator dyes for recording calcium activity is described. The postnatal SVZ contains tightly packed cells including neural progenitor cells and neuroblasts. Rather than using bath loading we injected the dye by pressure inside the tissue allowing better dye diffusion.
09:31
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
0 Views •
Cited by 15 •
2011
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification.
10:25
Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
0 Views •
Cited by 10 •
2012
We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.
11:29
Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
0 Views •
Cited by 52 •
2015
This video article describes experimental procedures to study long-term plasticity and its associative processes such as synaptic tagging, capture and cross-tagging in the CA1 pyramidal neurons using acute hippocampal slices from rodents.
08:46
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
0 Views •
Cited by 28 •
2014
In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.
FAQs
Rapid dissection and cutting in oxygenated physiological solution are central to maintaining the preparation after removal from the organism. Acute slices remain viable only for a limited period, while neurons retain much of their local synaptic connectivity and membrane function. This combination lets experiments examine circuit behavior under controlled conditions.
Preserved local synaptic connectivity allows researchers to study interactions among neurons within a microcircuit rather than examining isolated cellular properties alone. Because neurons also retain membrane function, experiments can address synaptic transmission, excitability, plasticity, and circuit interactions. The resulting observations connect cellular responses with the organization of nearby neural networks.
Researchers can combine patch-clamp recording, field-potential measurements, stimulation, imaging, and pharmacology to examine different aspects of slice function. Together, these approaches can assess synaptic transmission, neuronal excitability, plasticity, and interactions within a circuit. Using more than one readout helps relate cellular behavior to broader electrical or network-level responses.
Preparation begins with rapid dissection of the nervous tissue, followed by cutting the tissue in oxygenated physiological solution. The resulting sections are maintained outside the organism while they remain viable. Researchers then apply recording, stimulation, imaging, or pharmacological approaches during this limited experimental period to investigate neuronal and circuit properties.
Patch-clamp recording and field-potential measurements provide complementary ways to study activity in the preparation. Patch-clamp experiments can be combined with stimulation, imaging, or pharmacology, while field potentials provide another electrical measure of circuit responses. Selecting or combining these approaches helps researchers investigate transmission, excitability, plasticity, and circuit interactions.
Acute slices provide experimental access to nervous tissue while preserving much of its native local microcircuit structure and neuronal function. Researchers can therefore examine how disease-related changes affect synaptic transmission, excitability, plasticity, or circuit interactions under controlled conditions. The same preparation can support evaluation of candidate treatments through recording, stimulation, imaging, or pharmacology.