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Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
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Chapters
- 00:05Title
- 02:01Dissection of the Mouse Vomeronasal Organ (VNO)
- 03:37VNO Tissue Slice Preparation
- 04:37Immunohistochemistry of VNO Floating Slices
- 05:19Calcium Imaging of VNO Slices
- 06:23Representative Immunostaining and Calcium Imaging of VNO Slice
- 09:00Conclusion
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification.
Tags
Pheromone SensingMousePheromonesIntra-species CommunicationBiological FluidsUrinePheromonal CommunicationKin InteractionsHierarchical OrganizationsSexual InteractionsSurvival Of SpeciesVomeronasal Organ (VNO)Nasal CavityCartilaginous CapsuleSensory NeuroepitheliumVomeronasal Bipolar Sensory Neurons (VSNs)DendriteMicrovilliChemoreceptorLigandsVomeronasal Receptor FamiliesV1RsV2Rs
