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JoVE Journal
Neuroscience

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Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
 

Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation

Article doi: 10.3791/3311
December 6th, 2011

Summary December 6th, 2011

In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification.

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