Abstract
The endothelium is a dynamic integrated structure that plays an important role in many physiological functions such as angiogenesis, hemostasis, inflammation, and homeostasis. The endothelium also plays an important role in pathophysiologies such as atherosclerosis, hypertension, and diabetes. Endothelial cells form the inner lining of blood and lymphatic vessels and display heterogeneity in structure and function. Various groups have evaluated the functionality of endothelial cells derived from human peripheral blood with a focus on endothelial progenitor cells derived from hematopoietic stem cells or mature blood outgrowth endothelial cells (or endothelial colony-forming cells). These cells provide an autologous resource for therapeutics and disease modeling. Xenogeneic cells may provide an alternative source of therapeutics due to their availability and homogeneity achieved by using genetically similar animals raised in similar conditions. Hence, a robust protocol for the isolation and expansion of highly proliferative blood outgrowth endothelial cells from porcine peripheral blood has been presented. These cells can be used for numerous applications such as cardiovascular tissue engineering, cell therapy, disease modeling, drug screening, studying endothelial cell biology, and in vitro co-cultures to investigate inflammatory and coagulation responses in xenotransplantation.…
Embed Tool
Show this article's:
Embed code vs URL
An embed code allows you to place a JoVE video directly inside of your learning or training environment. Most learning management systems such as Canvas, Blackboard, and Moodle require an embed code for JoVE content to stream.
A URL allows you to place a link in programs such as PowerPoint or Microsoft Word which takes a user to a separate page where they can watch a JoVE video.
Most of the time, you will want to use the embed code. However, there are some environments that don’t support embed codes such as Google Classrooms and MS Teams. In those instances, you can use a URL to still provide access to JoVE videos.
What is an Embed Code?
Basically, it’s a snippet of code that you put on your website that displays as a video. An embed code pulls the video from the original source, allowing you to display a video without having to host the file on your website.
How to embed a video
STEP 1: COPY YOUR EMBED CODE
To generate the embed code, simply click the Copy button above. You can customize it by choosing a specific language for the video, or setting exact dimensions for the video player.
STEP 2: PASTE THE EMBED CODE INTO YOUR ENVIRONMENT
Embed codes can be used in most learning management systems (Canvas, Blackboard, Moodle…), organizational websites, and a host of other tools. The specifics of how to add in an embed code will depend on your environment.
Generally, it involves accessing a text editor, entering the HTML mode on the editor, and pasting in the embed code. Get more details on embedding into specific environments here.