Materials
Name | Company | Catalog Number | Comments |
LDLR-deficient mice (D2.129S7(B6)-Ldlrtm1Her/J) | The Jackson Laboratory | stock #002207 | |
Formalin | Sigma-Aldrich | HT5011 | |
Histology Molds | Ted Pella Inc | 27195 | |
Paraffin | Paramat | 19286-10 | |
Coated slides | Fisher | 12-550-15 | |
Solvent containers | Tissue Tek II | 4457 | |
Microtome | Leica | RM22S5 | |
Water bath | VWR | 80086-982 | |
Microscope | Olympus | BX41TF | |
Camera | Olympus | DP72 | |
ImageJ | NIH | www.rsbweb.nih.gov/ij/ | |
Processor | Sakura Finetek | VIP6-A1 | |
Processing cassettes | VWR | 18000-134 | |
Preassure Cooker | Nordic Ware | ||
Coverslip | Fisher | 12-545-M | |
PBS | Sigma-Aldrich | P3813 | |
Microtome blade | Thermo Scientific | 30-518-35 | |
Antifade mounting medium | Sigma-Aldrich | F-4680 | |
Xylene mounting medium | Sigma-Aldrich | 44581 | |
Aquous mounting medium | Sigma-Aldrich | I1161 | |
Mac-3 anbody | BD Pharmingen | 553322 | |
DAPI | Sigma-Aldrich | D9542 | |
F4/80 antibody | Abcam | Ab6640 | |
Alpha actin antibody | Santa Cruz Biotechnology | SC-32251 | |
Mayer’s Hematoxylin | Sigma-Aldrich | E4382 | |
Eosin Y | Sigma-Aldrich | MHS16 | |
DAB (3,3'-Diaminobenzidine) | Dako | K3468 |
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