Flow Cell Construction
Flow Cell Construction - Related Videos
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
07:23
Construction and Testing of Coin Cells of Lithium Ion Batteries
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Cited by 13 •
2012
A protocol to construct and test coin cells of lithium ion batteries is described. The specific procedures of making a working electrode, preparing a counter electrode, assembling a cell inside a glovebox and testing the cell are presented.
08:57
Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations
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Cited by 2 •
2016
Engineering and analysis of load bearing tissues with heterogeneous cell populations are still a challenge. Here, we describe a method for creating bi-layered alginate hydrogel discs as a platform for co-culture of diverse cell populations within one construct.
10:10
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Cited by 29 •
2009
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
11:08
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
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Cited by 4 •
2017
This article describes spectral cytometry, a new approach in flow cytometry that uses the shapes of emission spectra to distinguish fluorochromes. An algorithm replaces compensations and can treat auto-fluorescence as an independent parameter. This new approach allows for the proper analysis of cells isolated from solid organs.
FAQs
Flow rate and channel geometry determine how long substrates remain in contact with the surface, how effectively products are removed, and how uniformly liquid reaches the reaction area. These variables influence residence time, mixing, and exposure, so controlling them helps distinguish reaction behavior from transport effects and supports more reproducible enzyme assays, biosensor measurements, and surface-based studies.
Material compatibility helps limit unwanted adsorption and supports reliable contact with the liquids used in the experiment. Seals must maintain a closed reaction path around the channel and ports, because leakage can alter the delivered volume and disrupt controlled exposure. Together, suitable materials and secure seals improve measurement consistency and help preserve the intended biochemical environment.
Bubbles can interrupt liquid contact with the defined surface, while leaks change the intended fluid path and may reduce control over residence time. Contamination can introduce unwanted biochemical activity or interfere with measurements. Preventing these problems during assembly is therefore essential for stable liquid delivery, reliable surface exposure, and reproducible interpretation of reaction or sensor responses.
Assembly should account for the reaction channel, inlet and outlet ports, seals, and materials that contact the liquid or reaction surface. Their arrangement must support continuous delivery and removal without compromising the defined exposure area. Checking compatibility and the integrity of the assembled path helps reduce adsorption, leakage, bubbles, and contamination before measurements begin.
Flow cells support enzyme assays, biosensor development, chromatography, electrochemical measurements, and real-time microscopy. Their continuously refreshed conditions allow investigators to study reactions or molecular interactions while substrates are delivered and products are removed. This makes the approach useful when controlled surface exposure, ongoing measurement, or defined liquid movement is important to the experimental design.
A flow cell enables measurements under controlled residence time, mixing, and surface exposure rather than relying only on a static liquid condition. Continuous refreshment can reveal how molecular interactions or biochemical reactions behave as substrates arrive and products leave. In biochemistry, this supports real-time observation and more precise assessment of reaction or sensor behavior.