Nanoparticle Size Standards
Nanoparticle Size Standards - Related Videos
12:47
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
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Cited by 20 •
2012
Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
09:51
Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
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Cited by 34 •
2016
Size exclusion chromatography hyphenated with inductively coupled plasma - mass spectrometry (ICP-MS) is a powerful tool to measure changes in the abundance of metalloproteins directly from biological samples. Here we describe a set of metalloprotein standards used to estimate molecular mass and the amount of metal associated with unknown proteins.
08:21
A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions
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Cited by 4 •
2016
We describe a simple method for producing highly stable oligomeric clusters of gold nanoparticles via the reduction of chloroauric acid (HAuCl4) with sodium thiocyanate (NaSCN). The oligoclusters have a narrow size distribution and can be produced with a wide range of sizes and surface coats.
09:19
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
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Cited by 112 •
2021
We demonstrate how to use a novel nanoparticle tracking analysis instrument to estimate the size distribution and total particle concentration of extracellular vesicles isolated from mouse perigonadal adipose tissue and human plasma.
08:51
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Cited by 9 •
2013
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
FAQs
A defined size distribution provides a benchmark for judging whether an instrument or workflow reproduces the expected particle dimensions. This is important because a sample may contain particles spanning multiple sizes rather than one uniform diameter. Comparing the measured distribution with the reference helps reveal differences in sizing performance and supports more consistent interpretation across experiments.
Specified physical properties complement the assigned dimensions and help laboratories evaluate measurements under characterized reference conditions. These properties provide additional information for comparing how a method responds to the standard, rather than treating size as an isolated value. In bioengineering, that broader characterization strengthens confidence that reported particle dimensions are suitable for evaluating biological materials.
The same well-characterized reference material can be assessed with different sizing workflows, including dynamic light scattering, electron microscopy, and nanoparticle tracking analysis. Comparing each result with the known benchmark shows how closely a method measures the expected dimensions. Such comparisons help identify method-dependent differences and improve agreement when results are evaluated across laboratories.
Particle dimensions can influence transport, cellular interactions, stability, and biological performance. Consequently, reliable sizing is not merely a technical measurement issue; it affects how researchers interpret the behavior of engineered materials. Accurate characterization supports development and comparison of drug-delivery carriers, diagnostic probes, and biomaterials whose performance may depend on reproducible particle dimensions.
A laboratory can measure the reference material with its chosen sizing workflow, compare the result with the standard's characterized size distribution, and use the comparison to calibrate or validate the method. Repeating this assessment as part of quality control helps determine whether the workflow remains consistent. The same process also creates a basis for comparing measurements between laboratories.
Researchers should select a well-characterized standard with a defined size distribution and specified physical properties, then assess it using the intended measurement method. The observed dimensions can be compared with the known benchmark to evaluate method performance. This approach is useful before applying the workflow to research samples, because it establishes whether subsequent measurements are supported by a validated procedure.
A difference between the measured result and the standard's known benchmark can indicate that the sizing workflow requires further calibration or validation. It may also show that results from different methods should not be treated as directly equivalent without comparison. Identifying such discrepancies supports quality control, improves reproducibility, and helps laboratories report nanoparticle dimensions with greater confidence.
Nanoparticle Size Standards support characterization of drug-delivery carriers, diagnostic probes, and biomaterials. In each case, benchmarked sizing helps researchers compare formulations, assess measurement consistency, and relate particle dimensions to transport, cellular interactions, stability, or biological performance. Their use also strengthens method comparability during research and development conducted across laboratories.