Permeability Measurements
Permeability Measurements - Related Videos
13:42
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
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Cited by 19 •
2012
A spectrofluorometric protocol for the measurement of the mitochondrial permeability transition pore opening in isolated mouse heart mitochondria is presented here. The assay involves the simultaneous measurement of mitochondria Ca2+ handling, mitochondrial membrane potential and mitochondrial volume. The procedure for obtaining high-quality and functional heart mitochondria is also described.
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
14:20
Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
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Cited by 20 •
2014
Measuring the osmotic water permeability coefficient (Pf) of cells can help understand the regulatory mechanisms of aquaporins (AQPs). Pf determination in spherical plant cell protoplasts presented here involves protoplasts isolation and numerical analysis of their initial rate of volume change as a result of an osmotic challenge during constant bath perfusion.
07:03
An in vivo Assay to Test Blood Vessel Permeability
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Cited by 335 •
2013
We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.
07:23
Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans
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Cited by 5 •
2019
This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases.
FAQs
A gradient provides the driving force for transport across the test barrier. Concentration gradients are relevant when tracking solute movement, whereas pressure gradients can drive fluid passage. The measured response depends on which gradient is applied and on the barrier being tested, so identifying the driving condition is essential when interpreting transport behavior.
Flux reports the amount of material moving across a barrier under a particular experimental condition, while the permeability coefficient summarizes transport under defined conditions. This distinction helps researchers compare barriers or materials more meaningfully, because a coefficient connects the observed movement to the applied concentration or pressure gradient used during the assay.
Permeability can change when the barrier structure or experimental conditions change. Recording these factors allows researchers to determine whether differences arise from the material, membrane, cell layer, or tissue model itself, rather than from inconsistent testing conditions. Such comparisons support evaluation of barrier integrity and the performance of engineered bioengineering systems.
A typical workflow places the material, membrane, or biological barrier in a test system, applies a defined concentration or pressure gradient, and measures the resulting flux across the barrier. Researchers then use the gradient and measured transport to calculate a permeability coefficient. Keeping the test conditions defined makes results suitable for comparison.
The same measurement framework can examine transport through engineered membranes, biomaterials, cell layers, and tissue models. Comparing the resulting permeability values helps identify differences in barrier integrity and material performance. In bioengineering, this provides a basis for assessing how structural features or experimental conditions affect transport across distinct model systems.
Permeability data inform the design and evaluation of drug-delivery devices, filtration systems, and tissue-engineered constructs. In each case, transport through a material or biological barrier affects how the system performs. Measurements therefore help researchers assess whether a candidate construct or device provides the transport behavior required for its intended application.