Rat Mesentery Model
Rat Mesentery Model - Related Videos
18:30
Rat Mesentery Angiogenesis Assay
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Cited by 9 •
2011
Normal adult vascularized mammalian tissue that lacks physiologic angiogenesis and that has not been exposed to surgical intervention is used to study: (i) the initiation and development of angiogenesis following intraperitoneal administration of test agents; and (ii) modification of angiogenesis following systemic administration of selected test agents.
12:29
Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
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Cited by 19 •
2012
This article describes a simple model for stimulating angiogenesis in the rat mesentery. The model produces dramatic increases in capillary sprouting, vascular area and vascular density over a relatively short time course in a tissue that allows en face visualization of entire microvascular networks down to the single cell level.
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
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Cited by 3 •
2015
The modified Landis technique enables paired measurement of the hydraulic conductivity of individual microvessels in the mesentery of normal and genetically modified rats under control and test conditions using microperfusion techniques. It provides a convenient method to evaluate mechanisms that regulate microvessel permeability and transvascular exchange under physiological conditions.
07:26
Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel
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Cited by 49 •
2015
The FeCl3 induced thrombosis model in mice is described herein. A method to monitor thrombus growth by intravital microscopy observation on a mesenteric vessel and by blood flow measurement in the carotid artery is presented.
09:39
Creation of Reversible Cholestatic Rat Model
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2011
Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.
FAQs
Microscopy links cellular behaviors, such as leukocyte adhesion, with responses in arterioles, venules, and capillaries. This arrangement allows investigators to examine how inflammation-related cell activity corresponds to altered blood flow or vascular permeability within the same tissue. The resulting observations help relate local cellular responses to broader changes in microcirculatory function.
These vessel types provide distinct locations for evaluating microvascular responses. Studying them within the mesenteric network helps researchers determine how a controlled stimulus affects blood flow, leukocyte adhesion, permeability, or new vessel formation across the vascular bed. Comparing responses among vessel classes can clarify how tissue-level inflammation or angiogenesis develops through connected microvascular structures.
Inflammatory stimulation can be evaluated through changes in blood flow, leukocyte adhesion, and vascular permeability, whereas angiogenic stimulation is associated with new vessel formation. Separating these readouts helps distinguish immediate vascular and cellular responses from structural remodeling. The model therefore supports parallel investigation of inflammation and angiogenesis rather than treating every vascular change as the same process.
The tissue preserves microvascular networks in an intact environment while keeping them close enough to the surface for microscopy. Researchers can consequently observe responses as they develop across connected vessels, rather than relying only on isolated cellular measurements. This combination is useful for examining how inflammation, angiogenesis, and tissue remodeling influence one another during vascular change.
A typical workflow uses the mesenteric tissue as the biological preparation, applies a controlled inflammatory or angiogenic stimulus, and then examines the vascular network with microscopy. Investigators record outcomes such as blood flow, leukocyte adhesion, vascular permeability, or new vessel formation. The workflow can then compare untreated and treated conditions to assess how the stimulus changes microcirculation.
Microscopy can reveal blood flow through the microvascular network, leukocyte adhesion to vessels, vascular permeability, and the formation of new vessels. These observations provide both functional and structural information. Functional measures describe how the circulation responds, while new vessel formation indicates remodeling. Together, the outcomes help characterize how a treatment or stimulus affects living microcirculation.
Researchers can use the preparation when they need to examine how a drug or biomaterial influences microcirculation in living tissue. Controlled stimulation provides a context for testing whether the intervention changes inflammation, permeability, leukocyte adhesion, blood flow, or angiogenesis. Because several vascular outcomes can be visualized, the model supports comparison of treatment effects across related responses.
In biology research, the model connects observable microvascular behavior with processes relevant to vascular disease, including inflammation, altered permeability, angiogenesis, and tissue remodeling. It also permits evaluation of genetic factors alongside drugs or biomaterials. These applications help investigators study how different influences modify the structure and function of microcirculation in an intact tissue setting.