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Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
DOI:

12:29 min

May 20, 2012

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Chapters

  • 00:05Title
  • 02:06Rat Mesentary Exteriorization Model
  • 04:42Tissue Harvesting and Fixation
  • 06:06Tissue Immunolabeling: Colorimetric Labeling
  • 07:37Tissue Immunolabeling: Fluorescent Labeling
  • 08:27Results: Immunolabeling and Quantitative Analysis of Rat Mesentery Tissues
  • 11:35Conclusion

Summary

Automatic Translation

This article describes a simple model for stimulating angiogenesis in the rat mesentery. The model produces dramatic increases in capillary sprouting, vascular area and vascular density over a relatively short time course in a tissue that allows en face visualization of entire microvascular networks down to the single cell level.

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