Flow Cell Setup
Flow Cell Setup - Related Videos
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
12:04
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
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Cited by 75 •
2013
In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.
09:23
A Low Cost Setup for Behavioral Audiometry in Rodents
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Cited by 17 •
2012
A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
10:10
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Cited by 29 •
2009
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
FAQs
The pump determines how long a solution remains in the reaction or measurement chamber. Increasing flow rate shortens residence time, whereas decreasing it gives reagents or analytes longer exposure to the reaction zone, electrode, or measurement region. Adjusting this variable helps researchers balance processing throughput with reaction progress or measurement requirements while maintaining reproducible chemical conditions.
Channel dimensions and mixing influence how effectively reagents contact one another or an electrode. Their combined effect can improve mass transfer, meaning movement of chemical species through the flowing solution, and can therefore affect reaction or measurement efficiency. Researchers select and arrange these features to promote consistent contact throughout the chamber rather than relying on uncontrolled solution movement.
Temperature, pressure, and residence time are operating conditions that contribute to the efficiency of reactions and measurements in a flow cell. Controlling them helps keep the chemical environment consistent as solutions move through the system. This control is especially important when comparing runs, monitoring a process continuously, or linking a measured response to defined reaction conditions.
The chamber provides the location where the flowing solution contacts an electrode or passes through a measurement region. Its design connects fluid movement with the chemical event being studied, allowing controlled exposure under a defined flow rate and residence time. In electrochemical experiments, this arrangement supports reproducible contact between the solution and electrode during ongoing measurements.
Preparation requires arranging the channels, tubing, pump, and measurement or reaction chamber so that the sample can move through the intended path. The pump must deliver solution at a controlled flow rate, while the chamber must support the planned reaction or measurement. Researchers also establish the relevant mixing, temperature, pressure, and residence-time conditions before collecting results.
Chemists use this approach when they need continuous-flow synthesis, electrochemical experiments, spectroscopy, or online chemical analysis. The moving stream allows reagents to meet under controlled conditions and can reduce sample volumes while supporting real-time monitoring. It is particularly useful when reproducible processing and observation of an ongoing chemical process matter more than examining only a final sample.
Online monitoring provides measurements while the chemical process is moving through the cell rather than only after collection. Spectroscopic or other measurement arrangements can follow changes under controlled flow, temperature, pressure, and residence-time conditions. This makes it possible to relate observed signals to the ongoing reaction or analysis and to evaluate process behavior continuously.