Liver Kidney Binding
Liver Kidney Binding - Related Videos
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
08:29
SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
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Cited by 6 •
2019
Here, we present a protocol to enrich, isolate, identify, and characterize proteins modified by SUMO in vivo both from human hepatoma cells and liver tumors obtained from mouse models of hepatocellular carcinoma by using SUMO-binding entities (SUBEs).
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
09:15
Reliable and High Efficiency Extraction of Kidney Immune Cells
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Cited by 12 •
2016
Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.
FAQs
Molecular affinity determines how strongly a drug, biomolecule, or tracer associates with sites in hepatic or renal tissue, while concentration influences how extensively those sites become occupied. These effects can vary with local physiological conditions and tissue distribution. Interpreting both variables helps explain differences in organ exposure and supports more reliable pharmacokinetic assessments.
Tissue proteins, transporters, enzymes, and receptors can each provide binding sites in the liver or kidneys. Their involvement may affect where a compound accumulates and how it is processed or retained within an organ. Identifying the relevant component helps distinguish binding-related changes from broader differences in absorption, metabolism, or excretion.
Binding within hepatic and renal tissues can influence the amount of a compound available for organ-specific processing and removal. Consequently, measurements may help interpret clearance and identify changes associated with drug interactions. This information is clinically relevant because altered organ exposure can affect pharmacokinetic behavior and the assessment of potential toxicity.
Interpretation should account for molecular affinity, concentration, tissue distribution, and local physiological conditions rather than treating a binding value as fixed. Variation in any of these factors can change the observed association with hepatic or renal sites. Considering them together improves comparisons across experiments and strengthens pharmacokinetic or diagnostic conclusions.
Binding measurements provide information about organ exposure that can be incorporated into interpretation of absorption, distribution, metabolism, and excretion. In clinical pharmacology, these data support pharmacokinetic modeling and can contribute to dose selection. They are especially useful when researchers need to relate tissue association to clearance or evaluate whether exposure may raise toxicity concerns.
For diagnostic tracers and imaging agents, association with hepatic and renal tissues can affect how organ exposure is interpreted. Measuring this behavior helps researchers understand whether tissue distribution may influence the information produced by an imaging study. The resulting evidence can support development of more informative agents while also contributing to evaluation of organ-related safety.