Epithelial Cell Lines
Epithelial Cell Lines - Related Videos
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
03:24
Isolation and Culture of Murine Tongue Epithelial Cells: A Procedure to Isolate and Culture Tongue Epithelial Cells from Mouse Model
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2023
In this video, we describe the isolation of epithelial cells from a murine tongue. These cells can be further cultured to give rise to cell colonies.
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Cited by 7 •
2017
We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
08:00
Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo
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Cited by 6 •
2015
In this study, a detailed light microscopic technique was optimized for real-time observation and analysis of the motion of CPEC cilia ex vivo together with an electron microscopic method for ultrastructural analysis.
FAQs
Polarity creates distinct sides of an epithelial monolayer, while cell junctions connect neighboring cells and help maintain barrier properties. Together, these features allow researchers to examine whether pathogens interact differently with exposed and underlying surfaces, and whether infection alters epithelial organization. Maintaining these characteristics is therefore important when interpreting attachment, entry, barrier disruption, or tissue damage.
Researchers can follow the early stages of host-pathogen interaction by examining how a pathogen attaches to epithelial surfaces and gains access to cells. Surface receptors provide relevant interaction sites, while the organization of the cell layer supplies a model barrier. This approach helps separate effects on initial contact and entry from later effects involving replication or host responses.
Epithelial cells do more than provide a physical surface: they also participate in host innate immune responses. Infection models can therefore connect pathogen interaction with epithelial signaling and inflammation. Studying these responses helps clarify how epithelial barriers influence tissue damage and how local reactions may contribute to broader patterns of infection or transmission.
Adherent cultures provide a stable cell surface for observing epithelial behavior and pathogen interactions, whereas polarized monolayers more closely represent organized barrier properties with distinct cellular surfaces. The choice depends on the question being asked. A polarized model is especially useful when surface organization, junctions, exchange across the layer, or barrier disruption are central outcomes.
A typical study maintains the cells under controlled culture conditions, allows them to form the desired adherent or polarized organization, and then examines their interaction with a pathogen or treatment. Researchers assess outcomes such as attachment, entry, replication, innate immune responses, or barrier effects. The controlled setup supports comparisons across experimental conditions.
These cultures are useful when a treatment must be examined in the context of infected epithelial cells rather than only against a pathogen in isolation. Researchers can evaluate whether antimicrobial or antiviral treatments affect pathogen replication while also observing epithelial responses, barrier properties, inflammation, or tissue damage. This provides a broader view of treatment-related outcomes.
They can show how epithelial organization and barrier properties shape the consequences of infection, including inflammatory responses and cellular damage. Because epithelial surfaces influence contact with the environment, these models also support investigation of processes relevant to transmission. Such findings connect cellular events with the protective or disruptive roles of epithelial tissue during infection.