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Plating Primary Human Hepatocytes for Setting Up the All-Human 2D+ Hepatic Culture System (Video) | JoVE

A Physiologically-Relevant All-Human 2D+ Hepatic System for Drug Discovery

Article DOI: 10.3791/200769-v • 03:30 min • October 20th, 2023
October 20th, 2023 •
1, 2, 3, 4, 1, 1
1Institute of Regenerative Medicine, LifeNet Health, 2Biologics Division, LifeNet Health, 3LifeSciences Division, LifeNet Health, 4Research and Development, LifeSciences, LifeNet Health

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All-human Hepatic Culture System Drug Development Primary Human Hepatocytes Pharmacological Studies Toxicological Studies In Vitro Model Platforms Phenotypic Stability Experimental Reproducibility Thawing Plating Maintenance Two-dimensional Culture Techniques Three-dimensional Systems Attachment Plateability Seeding Density Functionality Long-term Culture Hepatocyte Colonies Hepatic-specific Marker Viability Architectural Integrity Albumin Levels Urea Levels Pharmacological Applications Toxicological Applications
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