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Journal / Neuroscience / 突触质膜和突触后密度蛋白采用不连续蔗糖梯度准备…
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Neuroscience

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JoVE Journal Neuroscience
突触质膜和突触后密度蛋白采用不连续蔗糖梯度准备
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突触质膜和突触后密度蛋白采用不连续蔗糖梯度准备

Article DOI: 10.3791/51896-v • 08:06 min • September 3rd, 2014
September 3rd, 2014 •
*1, *1, 1, 1
1Department of Pharmacology and Toxicology, University of Toronto
* These authors contributed equally

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本文将详细介绍与突触质膜上超速不连续蔗糖梯度相关蛋白的富集。随后的制备突触后密度蛋白也被描述。蛋白制剂适用于免疫印迹或2D DIGE分析。

Tags

神经生物学，第91，脑，突触，免疫印迹，超速离心，SPM，PSD
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